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The counts were performed using multi-channel configuration with a 40x objective and digital zoom of 2. When possible, 100 or more BrdU- or GFP-positive cells were scored for each marker per animal.
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The counting was performed using the built-in plugin of the Image J program.
The counting was performed using the Olympus Cell∧M imaging station.
The counting was performed using optical fractionator formula [35] and a 100× oil lens.
The count is performed using a standardized interview protocol.
The cell pellets were resuspended in 1 ml of PBS, and the total cell counts were performed using a Neubauer chamber.
The differential cell counts were performed using morphological criteria on cytocentrifuged preparations (Cytospin) after staining with Diff-Quick (Dade, Belgium).
The total leukocyte counts were performed using an automated haematology analyser (Cell-Dyn 3500, Abbott Diagnostics, Abbott Park, IL, USA).
The CD4 cell counts were performed using BD TruCount BD Biosciencee, San Jose, California) while HIV loads were determined using Amplicor HIV-1 Monitor Test (version 1.5) (Roche, Basel, Switzerland) from plasma stored at −80°C.
The total leukocytes and platelet counts were performed using a cell counter (HemaScreen 18, Ebram, BRA).
NC82 spot counts were performed using the Cell Counting Macro in the Metamorph software.
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