Sentence examples for the counter selection from inspiring English sources

There are at least two reasons possibly explaining the counter selection of gene overlap in Metazoa.

Modifications to the full-length CSFV cDNA were accomplished in E. coli DH10B (streptomycin resistant, StrepR) using the Counter Selection BAC Modification Kit Gene Bridgess, Heidelberg, Germany).

For the counter selection step, the bacteria were recovered in 10 ml LB in a 50 ml baffled conical flask and incubated for 4.5 hours in a 32°C shaking water bath.

For the counter selection step (see below), the bacteria were recovered in 10 ml LB in a 50 ml baffled conical flask and incubated for 4.5 hours in a 32°C shaking water bath.

It was important to establish purity, as the presence of cells in which integration had not occurred would contribute to a high background level of colonies when subsequently plated on the counter selection agents, FOA and FC.

For the counter selection the cultures were plated in the presence of 50 μM 6-methylpurine to induce a second homologous recombination step to recycle the selection marker and to eliminate integrated plasmid sequences.

Perhaps if you ask extra nicely, there's an under-the-counter selection of exorbitantly priced hockey sticks, bespoke cricket bats, rigorously flame-proof shell suits and ethically sourced trainers.

AGS cells were used as target cell for positive selections and GES-1 as negative cell for the counter selections.

Alternatively, a pIJ101-derived replicating plasmid combined with the counter-selection system CodA sm) was constructed for gene inactivation, which has a conjugation frequency as high as 0.52%.

Compared to conventional URA3 marker, in the counter-selection process, the new system generated 2 13 folds more colonies and the ratio of deletant was simultaneously elevated by 20 24%.

Thus, similar to the counter-selection cycles, the negative selection experiments did not reduce RNA binding to Aβ40 fibrils.