Exact(4)
DNA and predicted protein sequences were blasted with the corresponding sequenced genes and predicted protein sequences corresponding to strains 81-176, 81-116, NCTC11168, RM1221 and 269.97 using Blast with default parameters.
Each generated nucleotide sequence file is associated with a sample record containing extremely rich information on the environment of the corresponding sequenced Tara Oceans sample.
Transcriptome analysis tools included in the BioScope software package (Applied Biosystems) were used to map the corresponding sequenced reads against the mouse mm9 genome and the pig susScr2 genome.
Briefly, either a 20-ng aliquot of the ligation mixture or the corresponding sequenced plasmid was added to 40 μl of electrocompetent E. coli cells, and the mixed solution was transferred into an ice-cold 0.2-cm disposable cuvette (Bio-Rad Laboratories, HerCAles, CA, USA).
Similar(56)
Peptides detected in tryptic digest liquid chromatography tandem mass spectrometry (LC MS/MS) analysis of the purified PTEX sample are shown as black lines below the corresponding sequences.
To avoid diffusion, all fluorescent proteins were targeted to the ER by fusing them to the corresponding sequence motif (ER+HDEL motif67).
Multiple protein structural alignments were performed using MUSTANG (http://lcb.infotech.monash.edu.au/mustang), with the corresponding sequence alignment diagram prepared by using Chimera (https://www.cgl.ucsf.edu/chimera).ucsf.edu/chimera
Similarly, the cleavage sites for BsiWI and MluI enzymes were also crafted at the corresponding sequences of the human and mouse genes without changes in their amino acid sequences.
We introduced in the IFNα/βBP gene locus of the deletion mutant viruses the corresponding sequences encoding variant IFNα/βBPs lacking the ability to bind to the cell surface, generating the recombinant viruses VACVIFNα/βBPGAGmut and ECTV IFNα/βBPGAGmut (Supplementary Figure 2).
The initial experiment by Fire and Mello, showing that double-stranded RNA injected into worms would silence the gene with the corresponding sequence, was a clarion call to labs around the world, announcing a new method for investigating gene functions.
For EXP2 (a), PTEX150 (b) and HSP101 (c), secondary structure elements are shown as tubes (helices), lines (loops), and arrows (strands) above the corresponding sequence and are coloured as in Figs. 2a, 3a, 4a.
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