The amplified ORF were restricted with HindIII and XbaI and cloned between the corresponding restriction sites of the mammalian expression vector pcDNA3.1.
They were then inserted into the corresponding restriction enzyme sites in Renilla luciferase 3′ UTR of psiCHECK-1 to generate psiCHECK-SM.
The pCMMP Gagf vector was constructed by cloning a XhoI-BamHI fragment from pGag-GFP17 into the corresponding restriction sites of pCMMP KRAB18.
First, the AgeI-BamHI KRAB gene fragment from pCMMP KRAB was cloned into the corresponding restriction sites of pCMMP LacZ IRES GFP9, generating pCMMP KRAB IRES GFP.
The EcoRI-XhoI HIV-1 gag-pol gene fragment from pgag-pol18 was cloned into the corresponding restriction sites of pCMMP BlaM IRES GFP, generating pCMMP HIV-1 gag-pol IRES GFP.
A proportional trade-off is defined as a percentage change of the level of inputs/outputs so that the corresponding restriction is adapted with respect to the volume of the inputs and outputs of the DMUs in the analysis.
The same characterization applies to the corresponding restriction of problems in first order logic, giving an interesting new avenue for efficient inference when the number of objects is bounded.
The amplified genes were inserted into the corresponding restriction site of pETDuet-1 (Novagen; Madison, Wl).
The amplified genes were inserted into the corresponding restriction site of pACYCDuet-1 (Novagen).
The sequences of the restriction sites are underlined and the corresponding restriction enzymes are annotated.
The second part will propose the model of the emergency task planning and specify the corresponding restriction conditions.
