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Initial efforts to crystallize the RBD of RanBP3 in complex with Ran were hampered by the weak Ran-binding affinity of this domain, which we estimated by isothermal titration calorimetry (ITC) to correspond to a Kd of 14±0.3 µM (Figure 2; Figure S1 shows the corresponding experiment for full-length RanBP3), in agreement with a previous semi-quantitative study [18].
The corresponding experiment results are shown in Fig. 1.
The corresponding experiment settings are summarized in Table 8.
The corresponding experiment and simulation are validated the accuracy of presented empirical model.
The corresponding experiment proved that this probe can be repeated use above 10 times.
The approach is quantitively validated against the corresponding experiment reported by other researchers.
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Furthermore, we conduct the corresponding experiments to validate the theory.
The simulation results were then validated by the corresponding experiments.
Finally, the corresponding experiments are presented to validate the effectiveness and practicality of the proposed method.
And then the corresponding experiments are performed to qualitatively confirm the simulation results.
The corresponding experiments were performed and PSs were assembled in the different shapes regions.
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