Each of these parts contains only terms that are instances of the corresponding construct in the original source file.
To test whether attBs altered the level of activity of the enhancers, we electroporated in parallel a construct generated via Gateway technology (pSP72-B3-a-element-B4-bpFOG-B5::B1-NLS-LacZ-B2, pSP72-B3-eBra-B4-bpFOG-B5::B1-NLS-LacZ-B2) and the corresponding construct generated by conventional restriction enzyme cloning pSP72-a-element-bpFOG NLS-LacZZ, pSP72-a-element-bpFOG NLS-LacZ
Stably transduced cells expressing the corresponding construct were obtained by selection in medium with puromycin.
Second, the cross-loadings were compared to see whether the loadings with the corresponding construct were the highest.
The HB-002.1 HB-002.1[2]-Fc proteins were produced in CHO cells upon trandfection with the corresponding construct.
Parallel studies were simultaneously performed using the corresponding construct derived from the most closely related PLC isoform PLCδ1.
The grounded theory can be used to uncover information immersed in both context and cultures so as to develop the corresponding construct.
Pearson's correlations between the indirect and direct measures of the corresponding construct indicate whether indirect measures are well constructed and adequately cover the breadth of the measured construct [ 43].
To investigate the unusual processing pattern of L-Snord115, we subcloned the corresponding construct into the pcDNA 3.1 eukaryotic expression vector (fig. 2 A, sequence 1) and performed transient transfection experiments in HeLa cells.
In brief, Top10 bacteria transformed with the corresponding construct were grown in LB media until an OD600=0.6, expression was induced with 0.1 mM IPTG and cells further grown overnight at room temperature.
To generate the GST fusion proteins, the corresponding constructs were cloned into the bacterial expression vector pGEX-2T (Amersham Pharmacia Biotec Inc).
