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At the core of this variable outcome of infection and poor development of immunity lies the major parasite virulence factor and variant antigen, P. falciparum erythrocyte membrane protein 1 (PfEMP1).
Our humanization approach by resurfacing differed from the conventional one, as beside surface residues we tried to replace some residues in the core of variable regions as well.
The basic goal of the resurfacing is replacement of only surface murine residues, retaining the core of variable regions as undisturbed as possible to avoid possible conformational perturbances of the CDRs.
With unchanged amino acids in the core of variable regions, conformation of the antigen binding site is less likely to be disturbed, thus the specificity and affinity of the parent antibody should be maintained.
Additional highly conserved residues were identified in the core of structurally related human antibody variable regions.
The core of the language remains the same (variable definition, conditions, arithmetic and boolean expressions, etc).
Figure 12 illustrates a scenario on which a method from the core of BerkeleyDB calls methods from different variable features.
Before entering the core of the subject, let us introduce some basic variables.
The residues VH34 and VH73 belong to the 76 residues that form the "common core" of the IgG variable domains [ 26].
An overall, three-category measure of impairment was derived from the core impairment variables of gross motor function and IQ.
Several previous studies have indicated that diversification of the core and core variable components of the genome may play an important role in the adaptation of S. aureus to different hosts (Herron-Olson et al. 2007; Ben Zakour et al. 2008; Sung et al. 2008; Smyth et al. 2009).
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