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Considerable disagreement often exists regarding the conversion and activity of many oxidative coupling catalysts.
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At the same period, several biomethane potential (BMP) tests and SMA tests were conducted to monitor the conversion capacity and activity of the biomass at both conditions.
This exogenous expression system also enabled the investigation of the conversion activity of moPrP harbouring a P101L mutation (Figure 4A).
NecC was purified from the extract of T. discophora based on the conversion activity of 4-amino-4-deoxyarabinitol to nectrisine.
The conversion activity of wildtype 101P-moPrP was not significantly affected by chlorate treatment of the cells (Figure 4A,C).
The conversion activity of reactions containing mutant 101L-moPrP was significantly greater than those of wild-type 101P-moPrP, despite detection of lower 101L-moPrP levels (Figure 4B).
It was surprising that modification of GAG sulphation with chlorate did not decrease the conversion activity of moPrP.
In contrast the conversion activity of mutant 101L-moPrP was significantly increased following chlorate treatment (Figure 4A,D).
It has been previously reported that the conversion activity of 263K, a hamster adapted sheep scrapie strain, is decreased by enzymatic treatment to reduce the nucleic acid content [14] and recently suggested that nucleic acids do not contribute to the conversion activity of mouse adapted prion strains [40].
Unlike earlier reports, in which the heparinase III treatment did not affect the conversion activity of a hamster PrPC substrate seeded with 263K hamster adapted scrapie brain homogenate [14], we show that specific depletion of endogenous heparan sulphate inhibits the conversion activity of a mouse PrPC substrate when seeded with the M1000 mouse adapted human prion strain.
In order to improve the NOx conversion activity of the palladium catalyst, the effects of additives were investigated.
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