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After selection of the sample buildings and short-term field measurements from local climate throughout the year, the field data collected was used in simulation and comparison of the traditional and the contemporary sample buildings.
17 Data for the contemporary sample came from the Office of National Statistics.
No data regarding marital status were available for the contemporary sample.
Since we found limited population structure in the contemporary sample, only one haplotype per sampled individual was considered in all our demographic simulations.
Using census data from 1881, 1891, 1901 and 1911, we have accordingly standardised our incidence figures for the contemporary sample to the rates observed for the historical sample.
Written informed consent was sought from patients in the contemporary sample not currently in contact with the mental health services to interrogate their medical histories.
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To investigate the distribution of genetic diversity over the contemporary samples, an F-statistics based spatial analysis of molecular variance (SAMOVA) was performed using SAMOVA 1.0 [33].
Similarly, the contemporary sampling locations from Alaska, northern Canada and Greenland do not differ in allele frequencies or levels of genetic diversity when compared to pre-decline historic samples from Canada ([35]; data not shown) suggesting genetic stability over time at least in their northern distribution.
Nine alleles were not detected in the contemporary samples compared to historical samples.
In the contemporary samples, error rates were estimated by re-genotyping a subset (22%) of the samples.
Allelic richness was higher in the historical samples compared to the contemporary samples, indicating a population contraction.
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