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The constructs were checked by PCR for gapN or UTR1 genes, digestion with the proper RE combination and sequencing.
All of the constructs were checked by sequencing.
The constructs were checked by sequencing (Additional file 16).
All of the constructs were checked by enzyme digestion and then transformed to Synechocystis sp. PCC6803 cells [ 18].
The constructs were checked by sequencing and used as a bait to screen a random-primed Human Fetal Brain cDNA library.
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The construct was checked by sequencing.
The FoxO coding sequence within the construct was checked against the NCBI trace archive sequences for H. magnipapillata 105.
The construct was checked by sequencing the entire insert and used as a bait to screen a random-primed human brown adipocyte cDNA library constructed into pP6.
The construct was checked by digestion and sequencing.
To ensure that no errors were introduced by the PCR, the construct was checked with sequencing.
The construct was checked by sequencing the entire insert and used as a bait to screen at saturation a highly complex S. cerevisiae fragment genomic library constructed into pP6.
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