Your English writing platform
Free sign upSuggestions(1)
Exact(2)
The constructs in which only 12 11 or 12 0 were mutated displayed moderately reduced GFP expression.
The constructs in which all three motifs had been excised, all three motifs had been mutated, or only motif 12 5 was mutated all displayed severely reduced GFP expression.
Similar(57)
We consistently observed lower expression from the construct in which the EJC is targeted to AUG2 (≈25%).
Nevertheless, the use of the two AUG codons in this construct was similar to that observed from the construct in which the EJC is deposited in the coding region.
Notably, the YIU construct targeting luciferase was at least as effective as either the "classic" construct or the construct in which the target is embedded in a microRNA structure ("miR-30").
Our interpretation of these data is that, in the pK constructs, in which the CrtY and/or CrtI genes are under the control of the 35S promoter, leaf expression of one of the introduced transgenes interferes with leaf carotenogenesis.
Plants harboring the pP constructs, in which the CrtY and/or CrtI genes are under the control of the Pat2 promoter, do not show significant changes in leaf carotenoid content (Figure 2B).
We next used the luciferase assay to probe promoter activity of variants of the above constructs in which the putative binding sites for SRF, TFAP2, or EGR3 were altered.
Cells were transiently transfected with the indicated constructs in which the indicated amino acids of mANO1 ac) were replaced with the corresponding amino acids of mANO6 (as determined by the alignment shown in Figure 5 figure supplement 2).
Three of the four constructs in which the ELDKWAS sequence was incorporated into a β-turn (BT -like conformational site were recognized BT -likeF5 antibody.
Sadly, sometimes people don't learn from current events, let alone from their own history, and they become unable to question the intellectual constructs in which their careers are invested.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com