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The construction of the tissue microarrays was done using a tissue arrayer (Beecher Instruments, Inc., Sun Prairie, WI, USA) [ 48].
Details of the construction and evaluation of the microarray were described previously [7].
Independent colonies from both libraries were selected for the construction of the microarrays at the Australian Genome Research Facility (http://www.agrf.org.au/).au/
TH contributed to the construction of the microarrays, the microarray hybridizations and analysis.
A second version of the microarray is currently under construction, which contains an additional 500 clones to improve the representation of various biological pathways on the microarray.
The microarray was constructed from an EST-library of chicken testes and brain, and details of the construction and content of the microarray has been described elsewhere [44].
The collection of patient specimens and the construction of the tissue microarray (TMA) have been previously described [ 13].
Both the design of 70-mer oligonuclearotides for each gene and the construction of microarrays were made by CapitalBio Corporation (Beijing, China).
For the construction of the tissue microarray (TMA) fomalin fixed and paraffin embedded tissue was used as described previously [ 27].
The use of clinical specimens for the construction of tissue microarrays (TMAs) was approved by the ethical committee of the University Hospital of Basel, Switzerland.
Details on the construction of this microarray (AppChip1) are available on the Institute for Biological Sciences website [ 77]. cDNA synthesis and microarray hybridizations were performed as described [ 78].
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