Sentence examples for the construct generated from inspiring English sources

Since the Xho I site (CTCGAG) alone was successfully used as a loop to generate functional siRNAs (9), the construct generated by Xho I digestion and self-ligation is believed to be able to produce functional siRNAs.

Importantly, the expression of the M1X construct generated a clear protein band recognized by the anti-HA antibody (Fig. 3C).

Drug-resistant clones were screened for targeted integration by digestion of genomic DNA with AflII and MluI, followed by Southern blotting with a probe 5′ of the targeting construct generated with the primers BU1STHNF and BU1STHNR (Fig 2C).

Despite the presence of two ATGs followed by a stop codon at position 66, the M3 construct generated a protein, which was detected using the FLAG antiserum.

Overall we observed that the PEEK construct generated different stress/strain patterns in all areas of interest for this study.

Immunization of mice with the pEtxB BCL1scFv DNA construct generated high levels of Id-specific antibody and protected against lethal tumor challenge.

To test whether attBs altered the level of activity of the enhancers, we electroporated in parallel a construct generated via Gateway technology (pSP72-B3-a-element-B4-bpFOG-B5::B1-NLS-LacZ-B2, pSP72-B3-eBra-B4-bpFOG-B5::B1-NLS-LacZ-B2) and the corresponding construct generated by conventional restriction enzyme cloning pSP72-a-element-bpFOG NLS-LacZZ, pSP72-a-element-bpFOG NLS-LacZ

An ER retention signal was introduced into the genetic construct generating a p24 protein containing a SEKDEL amino acid sequence at its C-terminus.

The semicircular body that serves as a basement for the T-shaped construct generates heat uniformly and it is perfectly insulated on the outer perimeter.

The trapezoidal body that serves as a basement for the T-shaped construct generates thermal energy uniformly and it is perfectly insulated on the outer perimeter.

(C, D ) Site-directed mutagenesis of the p3.2mar-GFP construct generating p3.2mar(T → G -GFP results in loss of armor plate expression in transient transG -GFP.