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For the concentration response, final concentrations were 3, 10, 30, 100 und 300 ng/ml, respectively.
The concentration response curves were classified into four major classes (1−4) using previously published criteria.
IC50 values were determined by non-linear regression analysis of the concentration response data.
The concentration response relation was linear (R = 0.9992) within this range.
The concentration response relationship was plotted and fitted to a Hill's model.
Similar(55)
The concentration-response curve then has [100 % − IY] as maximum.
In these studies, it has been observed that the concentration-response curve is very sharp [30].
The EC50 estimations were conducted by non-linear regression analysis of the concentration-response curve.
The major criticism regarding the ECxx concept is the need of specifying a mathematical model for the concentration-response relation.
However, in these studies the concentration-response curve has not been found to be a strict step function.
Concentration allocation needs at least a vague idea about position and scale of the concentration-response curve.
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