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The velocity of the interface is deduced from a solute balance which involves the concentration given by the phase diagram modified by the Gibbs Thomson effect.
using a paradigm of successively increasing the concentration given to a single animal (Table 1).
Peptide kinase assays were performed with 2mUnits of each kinase as above, except MBP was replaced with the peptide at the concentration given in figure legends.
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Besides, 1/20 (v/v) was the concentration giving a strain response classified as sensitive.
The concentration giving 50% inhibition (IC50) was calculated by non-linear regression analysis.
The limits of detection (LODs) and quantification (LO Qs of the overall method were calculated as the concentration giving a signal to noise ratio of three and ten, respectively.
LOD is often defined as the concentration giving a signal equal to three times the baseline noise, and LOQ is similarly defined as the concentration giving a signal equal to nine times the baseline noise.
The method detection limit (MDL) was based on the concentration giving a peak approximately three times the matrix/instrument noise level.
The concentration giving 50% inhibition (IC50) was calculated by non-linear regression with the use of Graphpad Prism Version 5.0 for Windows (GraphPad Software, San Diego, CA, USA) (http://www.graphpad.com).
The concentration giving 50% inhibition (IC50) was calculated by non-linear regression with the use of GraphPad Prism Version 5.0 for Windows (GraphPad Software, San Diego, CA, USA) (www.graphpad.com).
Thereby, sampling of the dialysate at different time intervals, and subseqeuent analysis of the concentration, gives an idea of a substrate's concentration in the extracellular space over the observation time.
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