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We further examine how the composition of reference gene sets affects the accuracy of the statistic, and suggest methods for selecting appropriate reference sets for any genome, including bacteriophages.
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Others are likely more limited to specific clades and their detection depends on the composition of the reference genomes and the (presumably ancestral) presence of similar elements in clades related to these references and the phyletic extent of such homologs.
Besides, the decrease in the size of the effect is probably due to the fact that also the composition of the reference group (countries of origin) changed markedly over time, and some of the positive effects of parental education on student outcomes are now captured by the coefficients of countries of origin.
The strength of this study was the composition of the reference group.
Table 2 details the composition of the reference standard in terms of study design and database inclusion.
Furthermore, both the size and the composition of the reference set have an influence on the embedding quality.
These do not incorporate and use phylogenetic information and are affected by the composition of the reference alignment and the phylogenetic positions of query sequences.
Thus, it is not unexpected if the composition of the reference population used in a laboratory substantially influences the results for plasma creatinine.
Although the composition of the reference set is based on the functional assignment of the genes, but there is no specific algorithm to construct a reference set for individual species.
A more detailed analysis of this prospect in our current study, however, is hindered by the lack of deep genotyping information that would be required to optimize the composition of the reference population [ 49].
What is essential here is that the assessment be done by an objective third party who knows the composition of the reference sample that is unknown to the experimenter.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com