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Length length of the complete element in bp; LTR 3′ and LTR 5′ their coordinates in the complete elements are indicated; insertion time this is the estimate in million years; TSD sequence of the target site duplication n/a Not available, T truncated, TSD target site duplication.
In addition, the complete element encoding the transposase that mobilizes Tetris and DAIBAM was not found.
We reconstructed the complete element BmCR1B from B. mori using the genomic fragments available in databases.
The more plausible scenario is a transfer in the R. prolixus genome, from an unidentified source, of the complete element followed by a deletion event that gave rise to MITE_185 element and a secondary lost of the complete element.
Possibly, due to their smaller size and perhaps larger frequency in the genomes, these fragments are amplified with preference to the complete element, if present.
The solo-LTRs and additional integrase domains we find in this species, were all identified by the sequence of the complete element in this species, and not by any of the element sequences in D. yakuba or D. erecta.
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To reduce the possible contribution of LTRs from complete LTR-RTs to the homogenization of nucleotide distances for LTRs alone, we recalculated the coefficient of variation for LTRs by removing all three LTRs from the complete elements.
Specifically, both LTRs of the complete elements were aligned by CLUSTALW2 with default parameters.
The complete elements – displaying specific signatures and isolated from the genome assemblies – enabled the identification of recently transposed or divergent elements, including those present at a single copy.
The complete elements are flanked by 5-bp direct repeats usually generated during the LTR-retrotransposon insertions, suggesting that they are originated from different replications events.
These sequences may therefore still play the important role of repressing transposition of the complete elements of the same family through transposase dilution or through a negative dominant repression by the truncated transposases.
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