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The comparative alignment between VACV G8R, human and yeast PCNA protein sequences was created using T-Coffee with manual manipulations to ensure that the secondary structure that was being mapped contained no gaps.
A further question concerns whether there might be a biological basis associated with the observed heat-map patterns generated from the comparative alignment scores.
cDNA from unaffected dog skin obtained by RT-PCR was sequenced to verify the exon boundaries inferred from comparative alignments with the human and mouse orthologs.
There are few gaps over one million base pairs in this comparative alignment.
Taken together these results suggest that variants in BRCT domains of non-BRCA1 genes can be functionally inferred using comparative alignments with the BRCA1 protein BRCT domains and the extensive functional annotation available therein.
To begin to address this, two further analyses were carried out relating to: a) possible expression patterns associated with FAexpTRL from the top10% (red zone) regions and b) the relation of the heat-map patterns generated from comparative alignments with that generated for segmentally duplicated FAexpTRL.
Agreement in comparative alignments between the current linkage and RH maps suggests either map might be used to identify positional candidate genes underlying this QTL.
We first determined 'optimal' sets of comparative alignments by maximizing the number of known ncRNAs, serving as positive controls, recovered by different prediction conditions (Text S1).
Clicking on these tracks will enable viewing comparative alignments as well as information about the co-ordinates of the alignment on both genomes.
The comparisons of the corrected phi1 and RB49 genomes were realized using the LAGAN program (Brudno et al. 2003), which permits comparative alignments of entire genomes on a nucleotide level, and the circular genome visualizations were generated using CGView (Stothard and Wishart 2005).
These RMP values were used to produce the comparative map alignments.
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