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The columns were checked as to ultimate and serviceability limit states following the ABNT NBR 6118/07 Brazilian standard.
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The tested columns were checked under the sameconditions; with the Kromasil C18 and Zorbax Rx C8 columns, the peaks of impuritieswere overlapped with celecoxib peak.
Once the analysis is finished, note that the "Analysis" column is unchecked for both models and the "Results" column is checked for both models, indicating that parameter estimates and model fit statistics are available.
The purification column was checked for any residual wash buffer by re-centrifuging at 16,000 × g for 1 min and transferred to a new 0.5 mL micro centrifuge tube provided in the kit.
Then, the overlap of the column histograms is checked measuring the separation of the two maxima.
After Sfi I digestion and fractionation using a Chroma Spin-400 column (BD Clontech, USA) the fractions were checked using agarose gel and pooled into three tubes in a size-dependent manner (large, medium and small PCR products).
Note that in the list of models to be analyzed, the first column, "Analyze," is checked for both models.
Column yields was checked for each sample by analyzing Zn concentrations by quadrupole ICP-MS.
The labeled DNAs were purified on QIAquick columns (Qiagen) and the incorporation was checked by spectrophotometry.
Else, it's a data entry error somewhere along the line, to be checked one column at a time.
The columns are products.
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