Exact(35)
Class comparison was performed by using the Class Comparison module of BRB ArrayTools.
qPCR was performed on selected genes, to verify the class comparison analysis (Fig. 5B).
The class comparison at week 2 yielded 312 differentially expressed genes, 258 of which remained after normalization (84% upregulated; Table 6).
The Class Comparison Between Groups of Arrays Tool in BRB-ArrayTools v.3.8 software package ([21]; linus.nci.nih.gov/BRB-ArrayTools.html) was used to identify significantly changed genes.
These data were analyzed using the class comparison algorithm of BRB-ArrayTools (random variance model), which computes a paired t-test for each gene using the RMA-summarized log-intensities for Affymetrix U133A arrays.
On the other hand, the additional statistical method used to analyze the data, the Class Comparison Differences method, applied a two-sample univariate t-test to the unaffected control data set vs. the LGMD2A data set.
Similar(25)
Following the approach described previously with the class comparisons analysis, module analysis was applied initially to the training set of patients.
Statistical group comparison (Mann-Whitney, p<0.01) was applied to the class comparisons on the quality control (QC) genes present in the training set revealing 3,168 genes differentially expressed between S. aureus-infected patients and healthy controls.
The simulations thus suggested that only a negligible proportion of the information relevant to the question addressed would be missed in the class comparisons, and provided a high confidence toward the differentially expressed genes identified.
The GO class comparison yielded 11 categories significant at the nominal 0.005 level of permutation tests.
This is in contrast to the successful class comparison and prediction achieved with a publicly available cancer dataset [5].
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