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Figure 5C captures the ability of the CIA method to detect both collective and individual cell invasion, given appropriate stimulation.
Also, we noted that the variation and confidence levels associated with the CWA method were inferior to those of the CIA method (more discussion later).
Confocal microscopy images suggest that cells move forward through Matrigel™ in the CIA method, but remain attached to substrate (underlying dish).
Perhaps this was due to the less constrained range of motility that is associated with the matrix-free CWA technique, compared to a more constrictive microenvironment presented to cells in the CIA method.
Notably, the rates of wound-healing measured using the CIA method, in the presence of the Matrigel™ overlay, were significantly (P < 0.001) lower than those obtained from the traditional CWA method for the three cell lines tested.
Figure 5B is a summary of results obtained from DLD-1 cells, in the presence or absence of LPA at various times over the course of 24 h, using the CIA method.
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Given its ability to both determine pseudo-realistic invasive cell behavior in vitro and capture subtle differences in cell motility, we propose that our CIA method may shed some light on the cellular mechanisms underlying cancer invasion and deserves inclusion in further studies.
We introduced LPA in our CIA method in order to determine whether this assay can also be used to distinguish and quantify individual versus collective cell motility.
Sci. was funded by the CIA". Chomsky added that the CIA later went across the street to Harvard.
CIA Director Michael V. Hayden acknowledged the destruction of the tapes in a message distributed to the CIA workforce.
The head of the CIA, Michael Hayden, was blunter.
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