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After trypsinizing the cell, cells were washed in phosphate buffered saline and resuspended in 1% bovine serum albumin plus fluorophore-conjugated primary antibodies and incubated for 30 min at room temperature.
Before plating the cells, cell culture dishes or plates were coated with rLN511E8 (0.5 μg/cm2).
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If a sufficient number of MACs have assembled on the cell, cell death eventually occurs.
After all, the cell, cell membrane, and all the activities the cell engages in occur at levels too small for the naked eye to see.
Validation of the cell-cell adhesion protocol.
The cell-cell communication is most likely due to stress.
"We are interested in the dynamics of the cell-cell interaction," Bhatia says.
Conversely, the N terminus targets CagA to the cell-cell junctions.
The cell-cell signaling is a feedback, or survival, mechanism that counteracts changes in the physicochemical environment.
However, µtissues cultured on stiff substrates mimicking fibrotic microenvironments retained focal adhesions near the cell-cell interface, potentially to reinforce the cell-cell junction in response to excessive forces generated by myofibrils in stiff microenvironments.
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