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Recently the cDNA coding for anti-lipopolysaccharide factor (ALF) has been identified from the Indian mud crab, Scylla serrata and has been named S. serrata anti-lipopolysaccharide factor (SsALF).
After 24 h cells were cotransfected with 125 ng of plasmids containing the cDNA coding for the different constructs of MMP-2 and with 125 ng of plasmids containing the cDNA coding for HLA-A*0201 HLA-A*0201
Indeed, the presence of several EcoRV sites within the cDNA coding for AtHMA6 prevented us from using the Gateway-compatible strategy.
As expected, transient transfection of MCF-7 cells with the cDNA coding for ACSL4 resulted in three-fold protein levels as determined by Western blot (Fig. 2A).
The 36B4 probe corresponds to the region comprised between bases 683 and 842 of the cDNA coding for the human acidic ribosomal phosphoprotein P0 (NCB accession number M17885).
The cDNA coding for human Praf2 was obtained from the IMAGE consortium and cloned in pcDNA3 with a triple HA tag fused at its C-terminus.
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41, 66 The cDNA codes for a product of approximately 55 kDa (excluding the signal peptide sequence).
The cDNA codes for a polypeptide of 743 amino acids, which contains a cleavable signal sequence and six asparagine-linked glycosylation sites.
The expression vector contained the cDNAs coding for a potential fusion protein containing the GFP (EGFP), the 2A esterase of foot-and-mouth disease virus (FMDV), and HOXB4 with an additional N-terminal hemagglutinin (HA) epitope tag (eGFP2AHOXB4).
However, it is unclear whether the 5' sequence of this cDNA (coding for the N-terminus) has been identified since its gene has not yet been cloned.
The cDNA coding sequence for Ci-VSP [10] was a kind gift from Dr. Y. Okamura (NIPS, Okasaki, Japan).
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