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Presently, traditional biotechnology techniques have had a great influence on plant production by accelerating the breeding procedure and distributing disease-free seeds.
These promising results are a first step toward the potential use of the model as a support tool to design sunflower ideotypes adapted to the current worldwide ecological and economical challenges and to assist the breeding procedure.
During the breeding procedure, foreground selection was practiced from F1 generation till BC3F3 generation at each stage to select the plants having resistance alleles of the three target genes and only progenies having the resistance alleles were advanced for the next generation (Figures 1, 2 and 3).
For a detailed description of the breeding procedure (breeding occurred spontaneously and was not induced by hormone injections) of the tested specimens see Drescher [ 42].
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The proportion of the normal individuals decreased gradually to the tenth generation, indicating the feasibility of the selective breeding procedure.
Understand the breeding process.
The finding of similar fear acquisition in both groups suggests that the selective bidirectional breeding procedure per se had no influence on their general abilities to build fear memories in the fear conditioning paradigm.
Phenotypic data were collected, within the standard breeding procedures of DLF.
This breeding procedure was followed until the recombinant line was bred to homozygosity and then subsequent mice were generated by crossing homozygous recombinant males to B6 females and genotyping progeny to ensure that all were heterozygous for the desired recombinant region.
Approximately 10 years after developing the APO-SUS and -UNSUS lines a second, independent breeding procedure was started that resulted in rats with features similar to those displayed by the original APO-SUS and -UNSUS rat lines (Ellenbroek and Cools 2002).
In case of the other deciduous mandibular teeth that were not evaluated in this study, longer breeding procedure is required to finish their growth and development and receive meaningful data [ 22].
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