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All brains were dried at 110°C for 24 h, and the brain dry weight (dw) measured.
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I think I've dated women who were like my mom and women who weren't, but"—he paused—"I'm not somebody who has to go home and talk about theoretical physics at the end of a day when I've already been wringing my brain dry".
I think I've dated women who were like my mom and women who weren't, but" — he paused — "I'm not somebody who has to go home and talk about theoretical physics at the end of a day when I've already been wringing my brain dry".
Although gangliosides are abundant molecular determinants on all vertebrate nerve cells (comprising ≈1.5% of brain dry weight) their functions have remained obscure.
Representing 60 70% of human brain dry weight, lipids are notably important for proper brain function.
DHA (omega-3) and arachidonic acid (omega-6), which are long chain polyunsaturated fatty acids essential for PlsEtn synthesis, comprise 8% and 6% of brain dry weight, respectively [ 1].
Besides high oxygen utilization, two more reasons make the brain most vulnerable to oxidative damage: first, its modest antioxidant defences and second, its highly oxidizable substrate, that is, lipids comprise 60 65% of brain dry weight (see 'Discussion').
We kept the brains on dry ice during transportation to University of North Carolina where we stored them at –80° C until further processing.
If studies require the levels of metabolites (lactate, creatine) and markers of oxidative stress (thiol redox) to be preserved as close as possible to the in vivo condition, then rapid freezing of brain tissue via decapitation into liquid nitrogen, followed by chiseling the brain out at dry ice temperatures is required.
Before derivatization for GC-EIMS analysis, the brain steroids were dried down under nitrogen together with the internal standard 16-dehydropregnenolone (50 ng).
Journalists: they phone you up, suck your brains dry in search of new ideas for their next piece.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com