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Seal of the bottles were checked before storage.
The bottles were checked for any sign of crowding.
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Twice a week bottles were checked; if they were completely anaerobic, oxygen was added up to 1%.
Before blinding (i.e., before application of the plastic cover), the bottles were stored for ≥3 months and the solution was checked visually for foreign bodies.
His job was to check that the bottles were clean.
The bottles were incubated at 37°C for 7 10 days and visually checked for growth every day.
The bottles were labeled and air-tight.
The bottles were not shaken or stirred.
However, the seal on each bottle must be checked for any signs of tampering to ensure water safety.
The bottles are incubated for five days.
Cap the bottle with your handy bottle capper, and repeat until all the bottles are full.
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