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Extending the biomass to include lipid products involved significant work.
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In the same pathways, we also corrected the FN predictions associated with the bioA, bioF, and bioB genes by adapting the biomass objective function to include biotinyl-5'-adenosine monophosphate (AMP) and blocked the ability of the gene product of the bioF2 gene to catalyze the synthesis of the precursor of biotin.
This representation is still fully sufficient to compute quantitatively precursor effluxes into biomass synthesis and to include a biomass synthesis reaction accordingly.
Schematic picture for the conversion of lignocellulosic biomass to ethanol, including the major steps can been seen in Figure2.
The biomass reaction was altered to include amino acids, nucleotides, co-factors, and macromolecules rather than their precursor metabolites.
M. perforata flowered frequently during the experiment (13 weeks) so the presence of flowers was noted, and the flowers were clipped to include in the biomass measurements.
The biomass precursors include water, polysaccharides, nucleotides, amino acids, riboflavin, sulfate, and the general species "lipid".
We have chosen to include biomass as a species in the Yeast 5 GEM.
As such, we have chosen not to include biomass-related RE in this research.
The definition of renewable energy was also altered to include burning of biomass from native forests.
Pyrolysates originating from the lignin fraction within the biomass include phenol, guaiacol, syringol and related aromatic hydrocarbons.
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