Sentence examples for the bccp from inspiring English sources

Contrarily to the rest of the protein domains involved in biotin-dependent carboxylation, we did not analyzed the evolution of the BCCP domain because of the limited phylogenetic signal due to its small size (~ 90 amino acids) and low sequence conservation, which make the BCCP phylogenies very sensitive to reconstruction artifacts.

The BCCP was purified by Ni-NTA column chromatography.

The BCCP domains from different species have varied structural organization to interact with their homolgous enzyme(s) [26], [27].

The BCCP domain of heavier α (accA) subunit interacts with three distinct heterologous proteins; BCCP-BC, BCCP-CT and BCCP-BPL.

Design of primers, expression and purification of BCCP: The BCCP of M.tuberculosis has not been annotated as an individual gene but was part of acetyl CoA carboxylase in NCBI database.

The BCCP was biotinylated at 37°C for 30 min and then streptavidin conjugated horseradish peroxidase (HRP) was incubated at 1∶5000 dilutions for 1 h at 37°C.

EcBirA efficiently biotinylated both the BCCPs but MtBPL selectively biotinylated its cognate substrate (MtBCCP87) alone and failed to biotinylate EcBCCP87 (Figure 3c).

Cells expressing the BCCP-ribosome construct (E. coli strain KLF203) have no detectable phenotype.

BC catalyzes the ATP-dependent fixation of CO2 to the BCCP-bound biotin, and thus the intermediate formation of carboxybiotin.

The biotinylation status of the E. coli BCCP (the sole biotinylated protein in E. coli) was monitored with streptavidin-HRP.

The modeled BCCP domain was docked manually onto the subunit A of dhMtb-BirA by using the crystal structure of BirA and BCCP complex from P. horikoshii OT3 (2ejf) as a template.