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For instance, neither larvae nor adults were detected by traditional methods in the autumn samples from La Llosa and Boadella Reservoirs as well as in both temporal samples from the Sant Ponç Reservoir.
For instance, all cases of R. monacensis, the etiologic agent of Mediterranean spotted-fever-like illness, were from birds trapped in May, none from the autumn samples.
CGA, which showed large decreases in the autumn samples compared to the spring samples by microarray, also showed similar effects by qPCR (Table 1; 1-way ANOVA, F 3,24) = 30.76, p<0.01).
In the Autumn samples, this occurred in the barrel room in the main brewery, but in Spring and Summer this moved to a newly built facility dedicated to sour beers.
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Although no significant differences are found between sexes, males from the autumn sample have a higher number of scratches on the shearing molar facets than the females.
In the spring sampling, three locations (La Baells, Sant Ponç and Boadella) were positive for dreissenid DNA, and all five locations were positive in the autumn sampling (Table 2, Fig. 1).
This pattern can be observed in four of the five locations analysed (Fig. 1) but only in the Boadella Reservoir the DNA presence was significantly higher in the autumn sample (Fig. 1).
One tree had no leaves and could not be included in leaf CF analysis, and the bark of this tree had died by the autumn sampling date.
For instance, a significant change during Winter was identified when the intercepts of the regression of Autumn samples against time with last day active as day 1 and Spring samples with first day active as day 1 differed with p<0.05.
An additional source of difference in the degree of wear between spring and autumn samples might relate to the food consumed by the animals that supposedly varies depending on the season.
The prevalence (≅ 65% flocks infected) and mean number of eggs fell in the corresponding autumn sampling, with a substantial number of animals passing no eggs, thus accounting for the significant differences in the very low mean egg counts of ewes at this time.
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