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The increase is accounted for by the augmented volume of blood plasma (the liquid part of the blood), which is caused by fluid retention, plus an increase in the total number of red blood cells.
Of note, the absolute numbers of CARcs in femurs of aged mice were slightly increased compared to young mice, likely due to the augmented volume of femoral cavities as a consequence of the gradual loss of trabecular and cortical bone density associated to ageing (Fig. 7d f).
The augmented volume was estimated by CBCT using the image processing software, graft outlines were marked manually on the scans.
While autogenous bone is usually regarded to be the gold standard, harvesting may be associated with morbidity and considerable post-operative resorption of the augmented volume [2].
The long-term stability of the augmented volume found with this material is probably due to its slow resorption rate [8].
In various studies, the bone substitute was found to promote bone regeneration and to allow for long-term stability of the augmented volume [3, 5 8, 31 33].
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The measurement system we recently devised may help to quantify the difference in augmented volume in the future.
The aim of the present study was to test the hypothesis that human recombinant bone morphogenic protein 2 (rhBMP-2) implanted in a slow release carrier of polylactic acid (PLA) can repair a non-healing defect in the rat mandible and maintain the thickness of an augmented volume.
The thickness of bone tissue was maintained in Group 2 whereas it decreased in Group 1 and was negligible in Group 0. It is concluded that the PLA implants with 96 μg rhBMP-2 were able to bridge a non-healing defect in the rat mandible and maintained the thickness of an augmented volume.
In contrast to established polymethylmethacrylate (PMMA -based cements, aPMMA -basedof the BoneSourcementsnt enapplicationicatiof theoughout an augmented volume.
However, the augmented bone volume has a tendency to decrease postoperatively.
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