Since the assignment of reference or non-reference allele depends on the reference genome, the assignment per se is not of biological interest.
Since the assignment of reference and non-reference allele depends on the reference genome, whether a SNP is skewed toward reference or non-reference allele per se does not have direct biological meaning.
To confirm the assignment of HIV subtypes, reference sequences obtained from the Los Alamos Sequence Database and each Gabonese sequence were aligned with the ClustalX program [ 13].
By quantifying all of the template strands in a reaction, this method provides an improvement for the absolute quantification of DNA copy number which could be particularly useful for the assignment of values to reference materials [ 38].
This allowed the assignment of 4,367 Atlantic salmon reference genome contigs (corresponding to 57 Mb of sequence) to at least one linkage group (Table 4; Additional file 5).
Although the functions of the proteins/unigenes still need to be further validated, the assignment of functions provides important reference for identification of the targets in the in vivo experiment.
In particular, the gender focal point system did not appear to meet its mandate, in part due to incompleteness in the assignment of focal point responsibilities (terms of reference were often unclear) and the limited authority of the focal points.
Within the modeled area, the assignment of this BC was with reference to α-HCH production as well as disposals sites in Bitterfeld/Wolfen.
Indeed, to build sound final groups, we limited the assignment of a sequence to a reference group if the E-value was less than a threshold of 10-10 [Additional File 3].
MS/MS, however, cannot define the position of phosphate groups and, thus, the assignment of some metabolites, for which no references were available, remains tentative.
Recent efforts to identify RNA editing events in the human transcriptome using deep sequencing approaches have indicated that many of the identified RNA DNA differences could be explained by errors in sequencing or mapping errors in the assignment of RNA-Seq reads to the reference transcriptome.
