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In order to benchmark the assigned functional linkages in this study, three different reference sets were used.
The list contains chromosomal coordinates and RE sequence features resulting in the assigned functional grade.
Third, we identified only two out of the 28 human-specific "fixed" substitutions in the assigned functional transmembrane region.
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A general limitation in studying the origins of protein domains is the lack of assigned functional domains for about half of the residues in known proteins in eukaryotic species.
From information in the literature, we assigned functional categories and subcellular localization to the annotated complexes.
Using the available GSL profile information, the underlying allelic state at each of the eight genes assigned functional or non-functional, based on presence or absence of different GSL structures as well as the relative abundances of different structures, that is, based on the GSL profile of the accession.
The unigenes were assigned functional annotations, domains, metabolic pathways, Gene Ontology (GO) terms, Plant Ontology (PO) terms, and MIPS Functional Catalogue (FunCat) terms.
While three of the identified gene duplicates were annotated as mobile elements (retrotransposons or SINE element), the majority of the genes could be assigned functional annotation based on a manually curated homology search to UniProtKB/Swiss-Prot for those genes found to be duplicated.
All sequences have been uniformly annotated and assigned functional descriptions.
The analysis therefore assigns functional relevance to individual genetic interactions while also revealing global genetic architecture.
Variant annotation is the process of assigning functional information to DNA variants.
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