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In order to maximize the complementary nature of the assembly algorithms, we developed a meta-approach that reconciles the assemblies produced by either EULER-SR [1] or Velvet [2] and Newbler.
However, the assemblies produced by current algorithms are often incomplete.
The assemblies produced were compared to the reference sequence of Acinetobacter[ 11], and differences between the two versions were detected.
These results are almost identical to those shown for the assemblies produced by MIRA and shown in Figure 1, which did not involve an initial Velvet/Oasis assembly.
In contrast to Kumar and Blaxter (2010), we observed much less dramatic differences between the assemblies produced by Newbler v2.3 and Newbler v2.5 (Additional file 7).
The assemblies produced 84 contigs for the B (average contig length of 30.2 kb) and 144 contigs for the O (average contig length of 17.9 kb) genomes respectively.
Similar(45)
LiCoO2 is formed epitaxially on LLT with the orientation relationships (1 1 0)LLT//(11¯20)LiCoO2 and [0 0 1]LLT//[¯4401]LiCoO2 for both assemblies, but amorphous regions are formed at the interface for the assembly produced with polished LLT.
The assembly produced by EULER-SR resulted in about 5000 contigs that were larger than 500 bp with the largest contig being 12.5 kb.
Strain NRS1693 showed ~74% coverage, and the assembly produced 546 contigs (Table 1).
Given the total assembly size and contiguity and the percentage of mapped back metagenomic reads, we selected the assembly produced with a k-mer size of 31.
968 isotigs (5.6% of the total isotigs produced in the assembly) produced trees that were able to be analysed based on these parameters.
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