Sentence examples for the assays started from inspiring English sources

The assays started from 2 to 5 μg total RNA samples that were size fractionated using a YM-100 Microcon centrifugal filter (Millipore) and the sRNAs (< 300 nt) isolated were 3'-extended with a poly(A) tail using poly(A) polymerase.

The assay started with 5 µg of total RNA sample.

The assay started with 5 µg total RNA pools containing equal amount of RNA from individual lung (n = 6 per pool), which was size-fractionated using a YM-100 Microcon centrifugal filter (Millipore, Billerica, MA), and the isolated small RNAs (<300 nt) were 3'-extended with a poly(A) tail using poly(A) polymerase.

Briefly, the assay started from 2 to 5 µg total RNA sample, which was size fractionated using a YM-100 Microcon centrifugal filter (from Millipore) and the small RNAs (<300 nt) isolated were 3'-extended with a poly(A) tail using poly(A) polymerase.

Briefly, the assay started with 5 μg of total RNA.

Briefly, the assay started with approximately 6 μg total RNA.

Briefly, the assay started with 8 μg of total RNA.

Lysins were standardized to 1 μM per well and the assay started by the addition of 100 μl of cell suspension, giving an initial OD600nm=1.

The assay started from forming sandwich immunocomplexes by mixing Ab-PNIPAAm conjugate (capture reagent) and Ab-AP conjugate (detection reagent) in 50% human plasma, spiked with PSA (antigen).

The assay started with incubating 2 10 μg of purified enzyme of Sso0660 with 2% azocasein in different buffers at 55°C for 1 h.

The assay started from 5 μg total RNA sample, which was size fractionated using a YM-100 Microcon centrifugal filter (Millipore) and the small RNAs (< 300 bases) isolated were 3'-extended with a poly(A) tail using poly(A) polymerase.