Sentence examples for the assay without from inspiring English sources

The MERS-CoV assay with the hot start RT has a LoD of 6.9 copies/reaction (95%% confidence interval (CI): 4.2 15.4 copies/reaction), whereas the assay without aptamer has a LoD of 15.5 copies/reaction (95%% confidence interval (CI): 9.3 34.7 copies/reaction) (Fig. 2a, b).

The coefficient of variation (CV) across an entire plate was 2 4%, whether measured as the variation of the assay without artemisinin (at about 2 absorbance units), or with a 8 uM∶20 uM artemisinin∶heme ratio (at about 1 absorbance unit).

Finally the cells were stimulated again with a continuous flow of epinephrine solution for 30 min. The three independently operated inlets allowed us to perform continuous perfusion of cells during the assay without any abrupt changes of shear stress or laminar flow perturbation inside the microfluidic chamber.

As a control, prior to the beginning of each assay an aliquot was taken and was incubated until the end of the assay (without the enzyme).

The assay without uremic solutes was used as control (100% reaction) and the absorbance of the reaction medium without HOCl was used as a reading blank.

In each tube, 1 ng/ml of Interleukin-7 (R&D Systems Europe, Abington, UK) was added, a technique that has recently shown to increase the sensitivity of the assay without modifying its specificity [ 20].

After incubation, the hydrolytic enzymes were heat-inactivated 98°C 10 min, stored at 4°C, and the samples were used directly in the assays without further manipulation.

For the comparison between the methods used to measure total T, lower differences in total T concentrations were reported with the RIA incorporating extraction or chromatography compared with the assays without this extra step.

Searching further for a simple calculation method of avidity indices independent of IgG concentration and sample dilutions, we observed a linear relationship between the absorbance values of the assays without and with denaturing washing conditions.

We performed the migration assay without the use of Matrigel, however, there was no up-regulation of NO-induced migration activity (data not shown).

It is possible that other studies blinded the lactate assay without reporting the fact, but this matter remains speculative in the absence of confirmation from the study authors.