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However, several reports have shown that conventional and real-time PCR assays were very specific for SARS CoV but lack sensitivity depending on the assay, specimen, and time course of disease.
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For PCR assay, specimens were processed by lysis method as described previously [ 22].
This enables identification of one or several most similar (and equivalently matching) sequence records for each target pathogen gene sequence that may be generated in the assay of every specimen.
During the assay, the test specimen and HRP-HBcAb conjugates are incubated simultaneously with the coated microwells.
Furthermore, the variation in the reported sensitivity and specificity of RT-PCR may be related to the lack of standardization of the assay and the specimen collection (6 ).
At the end of the assay (15 min) specimens with spiked PSA show significantly higher fluorescence intensity than 0 pM specimen.
Before the assay test, all specimens were removed from the HGF, and then, 500 μL of alamarBlue® dye was added, followed by incubation for 4 h.
Xpert® MTB/RIF assay: The assay detected 12 specimens as MTB with rifampicin- resistance.
Critical features affecting the reliability of the assay, including protocols heterogeneity, specimen, cell cycle, kinetics, study design, and statistical analysis, are hereby discussed.
The assay is rapid for specimen concentrations ≥1 mg/l and is easily tuned to achieve low quantification limits at high chromatographic resolution for lower concentrated samples.
The assay sensitivity for serum specimen was 350 copies per mL [7].
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