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Data from the assay on ABTS-scavenging activity were analysed by one-way anlysis of variance (ANOVA) followed by Duncan's Multiple Range test.
assess the impact of implementation of the assay on clinical outcomes by means of randomised controlled trials (one such trial is in progress: http://clinicaltrials.gov/show/NCT01770730; vii. assess cost-effectiveness in different settings and algorithms. viii.
The performance of the assay on the complete validation panel, including carriers of at least one non-addressed allele, is shown in Table S7.
Several modified beacons have been used to validate the assay on both cell-free extracts and purified proteins.
Objectives: To evaluate the performance of the assay on a larger number of cerebrospinal fluid (CSF) specimens from patients suspected of having viral meningitis.
The limit of detection (LOD) was estimated to be 0.516 ng/ml, which is comparable with the LOD of 0.707 ng/ml obtained by the assay on a conventional titer plate assay platform.
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TaqMan® MGB probes were provided together with corresponding PCR primers by the Assay-on-demand™ service (Applied Biosystems).
TaqMan® MGB probes were provided together with corresponding PCR primers by the Assay-on-demand™ service (Applied Biosystems, Forster City, CA).
For the detection of BIC and the corresponding normalizer RNA Pol II, total RNA were converted into cDNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystem) and quantified with the assay-on-demand Hs01374569_m1 (Applied Biosystems).
All probes and primers were designed by the Assay-on-Design service of Applied Biosystems.
Sequence-specific primers and probes were selected from the Assay-on-Demand products (Applied Biosystems).
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