Sentence examples for the assay itself from inspiring English sources
The substrate was very specific with low background, and the assay itself could be done in less than 10 minutes.
If the assay itself is flawed or inconsistent, the data are worthless.
Additionally, the assay itself is of interest because it can screen large numbers of nanovaccines in vitro, simulating the process of antigen presentation and preventing extensive use of animals.
But the problem is less about the assay itself and more about how we chose to use it.
Firstly, while the pMV-absorption proceeds via complex cellular and molecular mechanisms (Figure 3), the assay itself is simple and expeditious and thus can be easily implemented as a high throughput assay (Figure 4A).
A functional antibody assay is based on this notion (antibody dependent cellular inhibition, ADCI) [6], [12], but it has not been widely reproduced in other laboratories, and the relevance of the assay itself to clinical protection in humans has not been statistically validated either in prospective longitudinal studies or otherwise.
It is clear that, as for influenza vaccine strategies, primer designs need to be reviewed regularly in light of prevalent sequence variations and the configuration of the multiplexed assay itself needs to be informed by the relevant epidemiology of the region.
Underestimation of copy number concentration can have several origins, related either to the PCR assay itself, to the density value used for the gravimetric dilution, to the volume of the assay or to a combination of those three factors.
To identify common single-nucleotide polymorphisms that could interfere with the pyrosequencing assay itself, the PCR products were cloned and sequenced (Baseclear, Leiden, The Netherlands).
The very nature of the supershift assay itself also does not allow the re-use of antibodies, thus making this technique somewhat costly.
To further demonstrate the specificity of the nanoparticle assay itself, bivalent Anti-MUC1 was added to MUC1*1110-ecd-peptide-bearing nanoparticles, which as expected resulted in the pink to blue color change.
