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The assay buffer (pH 8.3) comprised potassium di-hydrogen phosphate (0. 1 M), di-sodium hydrogen phosphate (0.1 M), and zinc chloride (10.0 mM).
Sections were incubated with 18F-7B (300 μL/slide; 10 nM) in the assay buffer for 60 min at room temperature.
Seawater served as the assay buffer.
The reaction was terminated by aspiration of the assay buffer.
Incubation of LP-48-A in the assay buffer only and LP-49-A in the assay buffer containing Fe-cMyco was carried out for 2 hrs.
The reaction mixture was incubated with 2 µg of rNSs protein in the assay buffer.
To reduce photobleaching, an oxygen scavenger system was added to the assay buffer [42].
In addition, we incubated the cells alone in the assay buffer.
cMyco at a concentration of 50 µM was also added in the assay buffer for LP-48.
15 µl of the assay buffer with DAPK, at a concentration of 4 µM, were added to each well.
On each plate, the reference standards for the respective target antibody, appropriately diluted in the assay buffer, were included.
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