Exact(9)
After the 24-h exposure, cells were incubated for 3 h with 500 μg/ml MTT reagent or for 2 h with 100 μg/ml neutral red dye, depending on the assay being performed.
The principle of the assay being that plasmids containing origins are maintained in extrachromosomal form in transformed yeast cells [30].
The plates were checked for AHSV cytopathic effect (cpe), with the end-point of the assay being taken as the highest dilution that prevented AHSV cpe in the wells.
The assay being employed is the Bio-Rad Variant II high-performance liquid chromatography system (HPLC).
Specificity was defined as the number of samples testing negative with the assay being evaluated, reported to the total number of true nonvirological failures as per VLref.
Sensitivity was defined as the number of samples testing positive, using the assay being evaluated, reported to the total number of true virological failures as per VLref.
Similar(51)
The assay was performed in triplicate.
The assay was repeated in triplicate.
The assay was repeated 3 independent times.
The assay was performed at pH 5.0.
The cost of the assay is $35.00.
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