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Which possible problems can arise from the application of this protocol to real cases?
The main research questions were: Which possible problems can arise from the application of this protocol to real cases?
We describe the application of this protocol to a human embryonic kidney cell line (HEK293T) and perform basic quality control, reproducibility, and benchmarking analyses.
We thus intend to continue and to widen the application of this protocol in an effort to evaluate if this positive effect will be observed in a greater number of patients.
The concentration of MMC was determined according to the risk of the surgery (Table 1), the cases were MMC was not used are previous cases to the application of this protocol [6].
The application of this protocol permits the design of experimental studies with direct evaluation of SR Ca2+ in real time in various mouse models of cardiac disease, including transgenic animals harboring mutants of RyRs or other Ca2+ signaling proteins.
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The application of this cloning protocol to DNA shuffling results in a protocol that we call 'Golden Gate shuffling'.
This shows sufficient feasibility for the application of the protocol for in vivo imaging.
There was no significant difference in the application of the protocol between the two groups.
The application of the protocol and vocal range behavior were evaluated with respect to the variable of warm-up.
WT hearts persistently showed SR following the application of the protocol.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com