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The APCI vaporizer temperature and capillary temperature were 350 and 300°C respectively, with a discharge current of 5 kV.
The APCI source was then selected and carefully optimized to produce reproducible spectra of the target compound.
For these reasons, the Ion Max API source was used with the APCI probe in the negative mode.
The soft ionization promoted by the APCI source allows effective and wide-scope screening based on the investigation of the molecular ion and/or protonated molecule.
In the APCI source, nebulizer pressure, capillary voltage, dry heater, and flow rate of dry gas were set to 3.0 bar, 3000 V, 250 °C and 2.5 L/min, respectively.
The APCI source showed better sensitivity than HESI, moreover ESI and/or HESI spectrum generally contains abundant and extensive series of adducts ions with methanol and inorganic ions [24, 38].
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The APCI-MS spectra, in combination with UV spectra, permitted the correct identification of compounds in the food samples.
Approximately 70 intermediates were detected by the SVUV-PI-MBMS, and their assigned molecular formulae are in good agreement with those detected by the APCI-OTMS, which has ultra-high mass resolving power and provides an accurate elemental C/H/O composition of the intermediate species.
Acetic acid detection using the APCI-MS headspace technique was in general more variable.
The APCI-MS clearly underestimated the amount of acetic acid present in this instance.
Cone voltage refers to the low voltage bias applied to the sampling cone in the APCI-MS source during analysis.
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