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The antitoxin worked.
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Subsequent structural work with CcdA has shown that the antitoxin protein, CcdA, has an intrinsically disordered domain and that the protein binds to two partially overlapping binding sites in CcdB to relieve its inhibition of gyrase (De Jonge et al. 2009).
My supervisors began working with the Food and Drug Administration and other government offices to get the antitoxin shipped to Tennessee and to respond to a public health emergency then of unknown dimensions.
Magazines and newspapers around the globe clamored to report on the "antitoxin horses".
The New York Herald organized a public fund-raising drive to support the antitoxin stable.
The surviving patients began improving soon after receiving the antitoxin that had arrived that day.
And the antitoxin had appeared to stop the disease progression.
The antitoxin serum was then separated from the clotted blood and purified using chemical techniques developed by health department bacteriologists.
Rather than the toxin CcdB in (A), the antitoxin CcdA was correlated with cellular density via AHL concentration.
Toxin-antitoxin systems are typically classified according to how the antitoxin neutralises the toxin.
The antitoxin inhibits the toxin by downregulating its expression.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com