The clean reads of the samples were mapped to the reference genome, and obtained from the annotation information of each sample using the RSEM (v1.2.0) software package [ 29].
The annotated gene models were similarly searched for functional motifs, even though in some cases the annotation information was already available in the public dataset.
The clean reads of the samples were mapped to the reference sequence of Uncaria transcriptome data, and obtained from the annotation information of each sample using RSEM software.
At least, five groups could be detected in terms of the annotation information (Table S6).
The annotation information of amino acid sequences from A. luchuensis genome searched using BLAST.
On mouse over, a preview of the annotation information is displayed in a pop-up window, as shown for 2GPU in this example.
The annotation information of amino acid sequences from A. luchuensis genome (accession nos: BCWF01000001 BCWF01000044, Yamada et al. 2016) was searched using BLASTP against the Swiss-Prot and TrEMBL protein databases from Uniprot (e-value cut-off 1e-5).
Should the annotation information for these SNPs held in common be counted twice (summation approach) or only once (union approach)?
Based on the annotation information, we generated genome-wide human and mouse cDNA and shDNA E3 collections ready for functional screening.
In general the genomics community has placed high value on the annotation information such that publication policies typically require metadata to be deposited concomitantly with assay measurements.
BLAST searches were also used to increase the annotation information.
