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Made from chenin blanc using the "ancestral" method, where the fizz-giving second fermentation takes place in the bottle without yeast or sugar being added to the still wine as it is in Champagne.
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We employed the ancestral area method from Bremer [ 39] to infer the direction and sequence of island and mainland colonization of C. flaveola in the West Indies.
Authors' response: Given the high computational demands on the ancestral reconstruction method (more than a week of wall-clock time), we find it impractical to test multiple root positions.
Among the six models tested in biogeographic inference, the ancestral area reconstruction method using the likelihood dispersal-vicariance analysis with the founder parameter (DIVAj) had a higher probability of being the best model for each of the three scenarios tested (Table 3).
All the ancestral area reconstruction methods identified Asia as the area of origin for Mycalesis, Culapa and Mydosama.
The estimated number of genes in 250 kb was used as the maximum distance between two matches in the Arabidopsis genome, since only the gene order, instead of the kb, was available as a position along the ancestral genome (see Methods).
Although the origin of the subgenus could not be inferred with this method, the ancestral biogeographical areas of most major clades are placed in this region.
The most frequent haplotype in the corresponding haplogroup was utilized as the ancestral haplotypes (therefore this method does not take into account error in the choice of ancestral haplotypes in the genealogy).
A P1 lysate was then made from that mixture of insertion mutants, and the lysate was used to transduce the ancestral strain, REL606 (see Methods for details).
It was previously shown that the distance based Bayesian method of the ancestral amino acid sequences inference is just as accurate in ancestor reconstruction as the method of maximum likelihood, differing favorably from it in fastness and more modest resource requirements [ 60].
Use of microlepidopteran hosts was recovered by both methods as the ancestral condition for the Rogadinae, but only with a low APP with the Bayesian method.
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