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The political implications and the analytical setup provided by the sustainability paradigm have had wide influence on science, politics, and economics.
The potential of the analytical setup was demonstrated on human follicular fluid (hFF) representing a typical complex human body fluid with clinical implication.
The experiments rely on a reversed flow injection analysis system especially designed for decoupling the analytical setup from environmental pressure for in situ applications.
Samples were quantified by direct flow injection analysis using the analytical setup described by Liebisch et al. [42], [43].
Samples were quantified by direct flow injection analysis using the analytical setup and the data analysis algorithms described by Liebisch et al. [42].
Figure 3 shows a schematic view of the analytical setup.
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The advantage of this analytical setup is its improved ability to detect genes with consistent expression differences across taxa, which may have been overlooked due to their mild expression change.
Using this analytical setup, we report on the analysis of oligosaccharides in urine samples of fucosidosis, α-mannosidosis, GM1-gangliosidosis, GM2-gangliosidosis, and sialidosis patients.
Despite its implementation, application of this analytical setup is not straightforward; it requires extensive calibration due to the effect of the electrochemical reactions occurring at electrode surfaces.
However, as discussed above, matrix effects are most pronounced with this approach; therefore, lower analyte loadings (method II or method III) can be beneficial, depending on the individual analytical setup and sample type.
Here, the capabilities of the program have been demonstrated by using it for the control of both a sequential injection analysis - capillary electrophoresis (SIA-CE) system with UV detection, and an analytical setup for studying the inhibition of enzymatic reactions using a SIA system with FTIR detection.
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