Prior to the proteomics analysis we verified the growth condition of S. aureus and the effect on the lifespan of C. elegans as there have been indications that the virulence and even the mode of infection of a bacterium is influenced by the type of growth medium they are cultivated on.
Genes found to be increased in uninfected males versus uninfected females are listed in Table 2. Using mRNA obtained in a separate experiment from the one used for the microarray analysis, we verified by qRT-PCR that the top five genes listed in Table 2 were more highly expressed in uninfected males compared to uninfected females (Table 2, Figure 2).
Using mRNA obtained in a separate experiment from the one used for the microarray analysis, we verified by qRT-PCR several of the genes shown in Figure 3. Cyp2e1 was the most highly expressed gene in this network with a fold increase of 1.8 (P = 9.4 × 10-4; Figure 3).
In the histology analysis, we verified that, differing from the liver and kidney, the lung of mice intoxicated with (PhTe 2 by s.c route did not present signals of severe lesions.
By introducing the finite element analysis, we verified the consistency between the whiplash test, the calibration test and the simulation results of them.
In the multiple linear regression analysis, we verified that the BODE index was a predictor of health status overtime.
From the results of an accessibility analysis, we verified that the developed system, V3DM+, can be implemented on real projects.
To confirm the correctness of the Fourier analysis we verify good agreement between the numerically computed on-axis image irradiance and this Gaussian approximation.
In a third analysis, we verified whether the introduction in the UK of new quality outcome framework guidelines over the study period, concerning the recording and treatment of diabetes and chronic kidney disease, 34 35 had affected the accuracy of both diagnoses of acute kidney injury and chronic kidney failure in the CPRD.
In an additional linear mixed model analysis, we verified the absence of carryover effects.
