A total of 62% (N=2195) of registered patients were successfully linked to at least one HES record and no ethnic group was recorded in HES for 24% (N=517) of the linked patients; thus, 1678 (47.5%) patients' ethnic classification derived from Nam Pehchan and/or SANGRA included in the analysis were validated using HES.
The microarray analysis was validated using qPCR to compare the mRNA levels of several selected ISGs in mock and infected cells (Additional file 8: Figure S6 (i)).
The immunohistochemical analysis was validated using positive controls (tonsil) and negative controls (by omitting the primary antibody).
The scaling analysis was validated using detailed numerical simulations for different enclosure geometries and outside diurnal climate conditions.
The microarray analysis was validated using qRT-PCR for 4 genes; their differential regulation was confirmed in all cases.
Expression of selected genes from the microarray analysis was validated using quantitative real-time PCR (qPCR), as follows.
Based on this knowledge, the HT analysis was validated using transgenic tobacco with altered lignin, and we demonstrate that it is a reliable and reproducible method for revealing differences in saccharification.
The microarray analysis was validated using qPCR, which showed an approximate 35-fold increase in the CD40 and TAP1 gene expression by 24 hpi (Additional file 8: Figure S6 ii)), consistent with the gene enrichment analysis indicating the presence of antigen-presentation pathways at 24 hpi.
The method of analysis was validated using the SRM-1570 cereferenceeference material (spinach), supplied by the National Institute of Standards and Technology USAA).
The miRNA expression analysis was validated using the TaqMan® MicroRNA Assay Kit (Applied Biosystems, Foster City, CA).
Analysis was validated using PlasmaCAL multi-element QC standard #1 solution (SCP Science, Canada).
