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As the determination of a cutpoint may be unstable with respect to perturbations of the data, this analysis was confirmed using non-parametric bootstrapping, by repeating the original analysis 2000 times using samples drawn from the original distribution with replacement.
The sampling adequacy of the food group variables for factor analysis was confirmed using the Kaiser Meyer Olkin measure.
Analysis was confirmed using the TP53 Mutation Handbook - the last release of the UMD_p53 database, that collects information on the TP53 status in cell lines published between 1989 and 2008 [ 19].
Our epistatic analysis was confirmed using the esg TS-Flip-Out system, a method of selectively activating a ubiquitous Gal4 driver in all progenitor cells and their subsequent progeny [ 10] (Additional file 10).
The normality of all data used in parametric correlative analysis was confirmed using Shapiro Wilk tests.
The identification of the peak at 8940 m/ z, as complement C3a des-arg using MS/MS analysis was confirmed using an immunodepletion approach.
Nuclear integrity following AFM analysis was confirmed using propidium iodide exclusion and map repetition on the same nucleus (Supplemental Fig. S1).
Ploidy analysis was confirmed using FACS.
Microarray expression analysis was confirmed using independent methods (Fig. 1B D and S2).
Differential expression of selected genes observed by RNA-seq or microarray analysis was confirmed using RT-qPCR or Northern hybridizations.
The down-regulation of Scd-2 (stearoyl-CoA desaturase) and Abcg1 (ATP-binding cassette subfamily G member 1) observed using microarray analysis was confirmed using real-time PCR.
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