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The analyses started from BioNJ trees.
Firstly, we decided on the analytical strategy before the analyses started.
The analyses started with a random tree and was run for 5,000,000 generations.
The analyses started by reading the transcribed interviews to obtain a general impression and identify the preliminary themes.
25 The primary analyses were all pre-specified in a detailed statistical analysis plan approved by the Data Monitoring Committee before the analyses started.
In both the GOS and MetaHIT projects, the analyses started by removing highly similar sequences (95 98% identity), followed by several steps of protein clustering.
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According to the P-SaMI data pattern, the analyses start after a percentage of snapshots has been docked.
Finally, since asthma can be difficult to diagnose in small children, 38 we conducted the analyses starting follow-up at age five years, both for the full cohort and for the restricted cohort of children born in 1996 or later, to allow inclusion of the anti-asthma medication algorithm and additional adjustment.
Although the analyses start with strict neutrality (s = 0) in each argument, alleles for which s < 1/ N, where N is the effective population size, can be considered nearly neutral, in that the effects of drift outweigh the force of selection [ 11].
Follow-up for these analyses started on the date of NADC diagnosis and finished at the earliest of the patient's death, 1st February 2010 or 6 months after the patient's last clinic visit.
The period under observation for the 'rebound analyses' started from 30 days after the 3rd course of IPTi (10 months of age) until 24 months of age, and all participants who received at least one dose of study drug and were still being followed-up during that period were included in the analyses (table 5).
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